Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001309242.1	NP_001296171.1	17 - 18	3779	60
	XM_017025120.1	XP_016880609.1	19 - 20	3486	60
	XM_017025121.1	XP_016880610.1	19 - 20	3471	60
	XM_017025122.1	XP_016880611.1	19 - 20	3450	60
	XM_017025123.1	XP_016880612.1	19 - 20	3488	60
	XM_017025124.1	XP_016880613.1	18 - 19	3360	60
	XM_017025125.1	XP_016880614.1	19 - 20	3485	60
	XM_017025126.1	XP_016880615.1	14 - 15	2951	60
	XM_017025127.1	XP_016880616.1	17 - 18	2794	60
	XM_017025128.1	XP_016880617.1	18 - 19	2540	60
	XM_017025129.1	XP_016880618.1	17 - 18	1772	60
	XM_017025130.1	XP_016880619.1	16 - 17	1728	60
	XM_017025131.1	XP_016880620.1	15 - 16	2167	60
	XM_017025132.1	XP_016880621.1	14 - 15	2108	60
	XM_017025133.1	XP_016880622.1	12 - 13	1633	60
	XM_017025134.1	XP_016880623.1	13 - 14	1994	60
	XM_017025135.1	XP_016880624.1	7 - 8	650	60
	XM_017025136.1	XP_016880625.1	7 - 8	999	60
	XM_017025137.1	XP_016880626.1	4 - 5	544	60
	XM_017025141.1	XP_016880630.1	19 - 20	3752	60
	XM_017025142.1	XP_016880631.1	19 - 20	3752	60
GenBank mRNA	AB058686.1	-	7 - 8	1886	60
	AF418286.1	-	15 - 16	4012	60
	AF418288.1	-	16 - 17	3812	60
	AF418290.1	-	-	4316	60

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	BF354043.1	-	1 - 2			Chr. 17 - 75602915 - 75603309 [-] on Build GRCh38	60
GenBankmRNA	BX110524.1	-	3 - 4	251		Chr. 17 - 75602510 - 75610261 [+] on Build GRCh38	60

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	LLGL2, scribble cell polarity complex component	LLGL2	HGL, Hugl-2, LGL2	-27.8kb	Sense	Chr. - 75525080 - 75575209 on Build GRCh38
Downstream	RecQ like helicase 5	RECQL5	RECQ5	23.5kb	Anti-sense	Chr. - 75626845 - 75667202 on Build GRCh38