Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
PFKM
Entrez Gene ID
5213
Gene Name
phosphofructokinase, muscle
Gene Aliases
ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122
Chromosome Location
Chr.12: 48105278 - 48146404 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_000289.5 NP_000280.1 16 - 17 1685 70
NM_001166686.1 NP_001160158.1 18 - 19 1987 70
NM_001166687.1 NP_001160159.1 16 - 17 1539 70
NM_001166688.1 NP_001160160.1 15 - 16 1628 70
XM_005268974.1 XP_005269031.1 19 - 20 1884 70
XM_005268975.1 XP_005269032.1 19 - 20 2023 70
XM_005268976.3 XP_005269033.1 19 - 20 2066 70
XM_005268977.1 XP_005269034.1 18 - 19 1870 70
XM_005268978.2 XP_005269035.1 18 - 19 1783 70
XM_005268979.1 XP_005269036.1 18 - 19 1870 70
XM_011538487.1 XP_011536789.1 17 - 18 1725 70
XM_011538488.2 XP_011536790.1 16 - 17 1650 70
XM_017019467.1 XP_016874956.1 19 - 20 1953 70
XM_017019468.1 XP_016874957.1 18 - 19 1805 70
XM_017019469.1 XP_016874958.1 17 - 18 1777 70
GenBank mRNA AK126020.1 - 20 - 21 2340 70
AK126229.1 - 19 - 20 1937 70
AK303681.1 - 11 - 12 1032 70
AK308185.1 - 11 - 12 1008 70
AK312284.1 - 16 - 17 1567 70
BC000534.2 - 15 - 16 1515 70
BC007798.2 - 14 - 15 1402 70
BC012799.2 - 15 - 16 1515 70
BC013298.2 - 15 - 16 1515 70
BC021203.2 - 16 - 17 1537 70
CR541698.1 - 6 - 7 555 70
J05533.1 - 7 - 8 596 70
KU178251.1 - 15 - 16 1500 70
KU178252.1 - 14 - 15 1407 70
KU178253.1 - 15 - 16 1500 70
KU178254.1 - 4 - 5 345 70
M26066.1 - 16 - 17 1576 70
U24183.1 - 15 - 16 1828 70
Y00698.1 - 15 - 16 1519 70

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