The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001282585.1 | NP_001269514.1 | 10 - 10 | 1415 | 201 |
| XM_017001516.1 | XP_016857005.1 | 10 - 10 | 1421 | 201 | |
| XM_017001517.1 | XP_016857006.1 | 9 - 9 | 3048 | 201 | |
| GenBank mRNA | AK027269.1 | - | 10 - 10 | 1415 | 201 |