The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001113434.4 | NP_001106905.1 | 2 - 2 | 701 | 91 |
| XM_005256621.3 | XP_005256678.1 | 2 - 2 | 694 | 91 | |
| XM_006721514.2 | XP_006721577.1 | 2 - 2 | 694 | 91 | |
| XM_011523822.2 | XP_011522124.1 | 2 - 2 | 694 | 91 | |
| GenBank mRNA | AK056436.1 | - | 2 - 2 | 667 | 91 |
| BC010612.1 | - | 2 - 2 | 699 | 91 | |
| BC019938.1 | - | 2 - 2 | 689 | 91 | |
| BC052777.1 | - | 2 - 2 | 91 | ||
| BC053952.1 | - | 2 - 2 | 91 | ||
| BC132738.1 | - | 2 - 2 | 675 | 91 | |
| BC136630.1 | - | 2 - 2 | 675 | 91 | |