The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000318.2 | NP_000309.1 | 4 - 4 | 1535 | 155 |
| NM_001079867.1 | NP_001073336.1 | 3 - 3 | 1503 | 155 | |
| NM_001172086.1 | NP_001165557.1 | 5 - 5 | 1541 | 155 | |
| NM_001172087.1 | NP_001165558.1 | 3 - 3 | 1399 | 155 | |
| GenBank mRNA | BC000661.2 | - | 4 - 4 | 1308 | 155 |
| BC093043.1 | - | 4 - 4 | 1321 | 155 | |
| M85038.1 | - | 4 - 4 | 1342 | 155 | |
| M86852.1 | - | 4 - 4 | 1450 | 155 | |