The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001135592.2 | NP_001129064.1 | 5 - 5 | 445 | 112 |
| NM_001177413.1 | NP_001170884.1 | 4 - 4 | 369 | 112 | |
| NM_002954.5 | NP_002945.1 | 5 - 5 | 543 | 112 | |
| GenBank mRNA | AK225079.1 | - | 1 - 1 | 260 | 112 |
| AK225718.1 | - | 1 - 1 | 260 | 112 | |
| AK315695.1 | - | 5 - 5 | 260 | 112 | |
| AY952889.1 | - | 1 - 1 | 247 | 112 | |
| BC001392.2 | - | 1 - 1 | 249 | 112 | |
| BC066293.1 | - | 1 - 1 | 260 | 112 | |
| BM907473.1 | - | 2 - 2 | 112 | ||
| BQ276758.1 | - | 4 - 4 | 263 | 112 | |
| BQ421613.1 | - | 1 - 1 | 128 | 112 | |
| CK004647.1 | - | 5 - 5 | 419 | 112 | |
| CN344335.1 | - | 4 - 4 | 244 | 112 | |
| DA527860.1 | - | 4 - 4 | 345 | 112 | |
| DB505702.1 | - | 5 - 5 | 445 | 112 | |
| EU794671.1 | - | 5 - 5 | 260 | 112 | |
| M10939.1 | - | 4 - 4 | 210 | 112 | |
| S79522.1 | - | 5 - 5 | 260 | 112 | |
| X63237.1 | - | 1 - 1 | 247 | 112 | |