The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| GenBank mRNA | AB061546.1 | - | 3 - 3 | 231 | 132 |
| AB451391.1 | - | 3 - 3 | 158 | 132 | |
| BC071716.1 | - | 3 - 3 | 180 | 132 | |
| BC100031.1 | - | 3 - 3 | 184 | 132 | |
| DA338824.1 | - | 3 - 3 | 244 | 132 | |
| X73459.1 | - | 3 - 3 | 177 | 132 | |