The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001258017.1 | NP_001244946.1 | 5 - 5 | 642 | 180 |
| NM_001258018.1 | NP_001244947.1 | 5 - 5 | 724 | 180 | |
| NM_172374.2 | NP_758962.1 | 5 - 5 | 666 | 180 | |
| NR_047577.1 | 6 - 6 | 701 | 215 | ||
| GenBank mRNA | AJ880386.1 | - | 5 - 5 | 541 | 180 |
| AJ880387.1 | - | 5 - 5 | 518 | 180 | |
| AJ880388.1 | - | 5 - 5 | 580 | 180 | |
| AJ880389.1 | - | 6 - 6 | 578 | 215 | |
| BC026103.1 | - | 5 - 5 | 570 | 180 | |
| BC131625.1 | - | 5 - 5 | 555 | 180 | |