The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000600.4 | NP_000591.1 | 2 - 2 | 310 | 133 |
| XM_005249745.4 | XP_005249802.1 | 1 - 1 | 450 | 133 | |
| XM_011515390.2 | XP_011513692.1 | 3 - 3 | 643 | 133 | |
| GenBank mRNA | A09363.1 | - | 2 - 2 | 275 | 133 |
| AK298013.1 | - | 1 - 1 | 414 | 133 | |
| AK301141.1 | - | 2 - 2 | 305 | 133 | |
| BC015511.1 | - | 2 - 2 | 238 | 133 | |
| BT019748.1 | - | 2 - 2 | 189 | 133 | |
| BT019749.1 | - | 2 - 2 | 133 | ||
| CR450296.1 | - | 2 - 2 | 189 | 133 | |
| HY076725.1 | - | 2 - 2 | 133 | ||
| M14584.1 | - | 2 - 2 | 252 | 133 | |
| M18403.1 | - | 2 - 2 | 251 | 133 | |
| M29150.1 | - | 2 - 2 | 224 | 133 | |
| M54894.1 | - | 2 - 2 | 239 | 133 | |
| S56892.1 | - | 2 - 2 | 204 | 133 | |
| X04403.1 | - | 1 - 1 | 96 | 133 | |
| X04430.1 | - | 2 - 2 | 275 | 133 | |
| X04602.1 | - | 2 - 2 | 222 | 133 | |