The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_032338.3 | NP_115714.1 | 3 - 3 | 328 | 135 |
| GenBank mRNA | AK057947.1 | - | 2 - 2 | 241 | 135 |
| AK091568.1 | - | 3 - 3 | 328 | 135 | |
| BC006002.1 | - | 3 - 3 | 324 | 135 | |
| BC070232.1 | - | 3 - 3 | 320 | 135 | |
| BC107780.1 | - | 3 - 3 | 329 | 135 | |