Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: RPL23AP32
Entrez Gene ID: 56969
Gene Name: ribosomal protein L23a pseudogene 32
Gene Aliases: RPL23A_9_220
Chromosome Location: Chr.2: 54529222 - 54529841 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NR_002229.2		1 - 1	49	78
GenBank mRNA	BC096706.1	-	1 - 1	49	78
	BC096740.1	-	1 - 1	49	78
	BC098172.1	-	1 - 1	49	78

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	AA610335.1	-	1	184		Chr. 2 - 54529086 - 54529573 [+] on Build GRCh38	78
GenBankmRNA	AA618193.1	-	1	185		Chr. 2 - 54529086 - 54529518 [+] on Build GRCh38	78

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	chromosome 2 open reading frame 73	C2orf73		-145.5kb	Sense	Chr. - 54329961 - 54383673 on Build GRCh38
Downstream	echinoderm microtubule associated protein like 6	EML6		193.8kb	Sense	Chr. - 54723467 - 54972908 on Build GRCh38

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