Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
BRAF
Entrez Gene ID
673
Gene Name
B-Raf proto-oncogene, serine/threonine kinase
Gene Aliases
B-RAF1, B-raf, BRAF1, NS7, RAFB1
Chromosome Location
Chr.7: 140719331 - 140924764 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq XM_017012558.1 XP_016868047.1 10 - 10 1244 86
XM_017012559.1 XP_016868048.1 10 - 10 1244 86
GenBank mRNA AJ276310.1 - 1 - 1 63 86


Adjacent Gene Details

Gene Name Gene Symbol Alias Distance Relative Direction Location Chromosome
Upstream NADH:ubiquinone oxidoreductase subunit B2 NDUFB2 AGGG, CI-AGGG -79.0kb Anti-sense Chr. - 140696681 - 140706646 on Build GRCh38
Downstream mitochondrial ribosomal protein S33 MRPS33 CGI-139, MRP-S33, PTD003, S33mt 220.4kb Sense Chr. - 141006161 - 141014981 on Build GRCh38

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