Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: RIF1
Entrez Gene ID: 55183
Gene Name: replication timing regulatory factor 1
Gene Aliases: -
Chromosome Location: Chr.2: 151409883 - 151534200 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	XM_011511395.2	XP_011509697.1	35 - 35	9976	86
GenBank mRNA	AL117611.1	-	1 - 1	50	86

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	DA915741.1	-	1	368		Chr. 2 - 151479964 - 151480668 [+] on Build GRCh38	86
GenBankmRNA	DA519167.1	-	1	399		Chr. 2 - 151479933 - 151480501 [+] on Build GRCh38	86
GenBankmRNA	BJ998059.1	-	1	274		Chr. 2 - 151480058 - 151480657 [+] on Build GRCh38	86
GenBankmRNA	BM720953.1	-	1	613		Chr. 2 - 151479720 - 151480377 [+] on Build GRCh38	86
GenBankmRNA	BG572722.1	-	1	100		Chr. 2 - 151480232 - 151480890 [+] on Build GRCh38	86
GenBankmRNA	DA694257.1	-	1	377		Chr. 2 - 151479955 - 151480527 [+] on Build GRCh38	86

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	TNF alpha induced protein 6	TNFAIP6	TSG-6, TSG6	-99.0kb	Sense	Chr. - 151357592 - 151380048 on Build GRCh38
Downstream	nebulin	NEB	NEB177D, NEM2	3.2kb	Anti-sense	Chr. - 151485334 - 151734487 on Build GRCh38

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