Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: BACE2
Entrez Gene ID: 25825
Gene Name: beta-site APP-cleaving enzyme 2
Gene Aliases: AEPLC, ALP56, ASP1, ASP21, BAE2, CDA13, CEAP1, DRAP
Chromosome Location: Chr.21: 41167801 - 41282534 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_012105.4	NP_036237.2	9 - 9	5541	103
	NM_138991.2	NP_620476.1	8 - 8	5391	103
	NM_138992.2	NP_620477.1	8 - 8	5372	103
GenBank mRNA	AK023647.1	-	1 - 1	47	103

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	DA225894.1	-	1	184		Chr. 21 - 41278962 - 41279497 [+] on Build GRCh38	103
GenBankmRNA	AL710874.1	-	1	493		Chr. 21 - 41278654 - 41279395 [+] on Build GRCh38	103
GenBankmRNA	HY337045.1	-	1			Chr. 21 - 41278823 - 41279271 [-] on Build GRCh38	103
GenBankmRNA	AU138714.1	-	1	47		Chr. 21 - 41279099 - 41279776 [+] on Build GRCh38	103
GenBankmRNA	BU195358.1	-		41		Chr. - - [-] on Build	103

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	placenta specific 4	PLAC4	C21orf115, PRED78	-93.9kb	Anti-sense	Chr. - 41175231 - 41185239 on Build GRCh38
Downstream	family with sequence similarity 3 member B	FAM3B	2-21, C21orf11, C21orf76, ORF9, PANDER, PRED44	21.7kb	Sense	Chr. - 41304229 - 41357727 on Build GRCh38

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