Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: PDE3A
Entrez Gene ID: 5139
Gene Name: phosphodiesterase 3A
Gene Aliases: CGI-PDE, CGI-PDE A, CGI-PDE-A, HTNB
Chromosome Location: Chr.12: 20369245 - 20684107 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000921.4	NP_000912.3	16 - 16	6640	92
	NM_001244683.1	NP_001231612.1	15 - 15	5897	92
	XM_017019421.1	XP_016874910.1	16 - 16	9338	92
GenBank mRNA	AK095203.1	-	1 - 1	1149	92

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	BX093899.1	-	1	396		Chr. 12 - 20683055 - 20683868 [+] on Build GRCh38	92
GenBankmRNA	CD101794.1	-	1	524		Chr. 12 - 20682920 - 20683649 [+] on Build GRCh38	92
GenBankmRNA	CD678352.1	-	1	540		Chr. 12 - 20682906 - 20683534 [+] on Build GRCh38	92
GenBankmRNA	BQ440685.1	-	1	717		Chr. 12 - 20682725 - 20683604 [+] on Build GRCh38	92
GenBankmRNA	CB962781.1	-	1	411		Chr. 12 - 20683036 - 20683715 [+] on Build GRCh38	92
GenBankmRNA	BP425408.1	-	1	492		Chr. 12 - 20682954 - 20683510 [+] on Build GRCh38	92

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	uncharacterized LOC100506393	LOC100506393		-583.4kb	Sense	Chr. - 20014685 - 20098868 on Build GRCh38
Downstream	solute carrier organic anion transporter family member 1C1	SLCO1C1	OATP-F, OATP1, OATP14, OATP1C1, OATPF, OATPRP5, SLC21A14	11.3kb	Sense	Chr. - 20690505 - 20753386 on Build GRCh38

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