The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000441.1 | NP_000432.1 | 13 - 13 | 1704 | 111 |
| XM_005250425.2 | XP_005250482.1 | 13 - 13 | 1687 | 111 | |
| XM_017012318.1 | XP_016867807.1 | 11 - 11 | 1405 | 111 | |
| GenBank mRNA | AF030880.1 | - | 13 - 13 | 1704 | 111 |
| AK294388.1 | - | 4 - 4 | 583 | 111 | |
| AK298543.1 | - | 3 - 3 | 327 | 111 | |
| AK300604.1 | - | 4 - 4 | 399 | 111 | |