The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_030662.3 | NP_109587.1 | 7 - 7 | 1155 | 74 |
| XM_017026989.1 | XP_016882478.1 | 7 - 7 | 1210 | 74 | |
| GenBank mRNA | AK093125.1 | - | 4 - 4 | 1202 | 74 |
| AK126446.1 | - | 1 - 1 | 1460 | 74 | |
| BC000471.2 | - | 7 - 7 | 1155 | 74 | |
| BC018645.2 | - | 7 - 7 | 1097 | 74 | |
| BX538081.1 | - | 8 - 8 | 905 | 74 | |
| L11285.1 | - | 7 - 7 | 985 | 74 | |