The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001258307.1 | NP_001245236.1 | 7 - 7 | 928 | 68 |
| NM_207310.2 | NP_997193.1 | 7 - 7 | 1126 | 68 | |
| XM_005263840.2 | XP_005263897.1 | 7 - 7 | 1151 | 68 | |
| XM_005263842.2 | XP_005263899.1 | 7 - 7 | 953 | 68 | |
| XM_006712833.2 | XP_006712896.1 | 7 - 7 | 1163 | 68 | |
| XM_006712834.3 | XP_006712897.1 | 7 - 7 | 1103 | 68 | |
| XM_006712835.2 | XP_006712898.1 | 7 - 7 | 965 | 68 | |
| XM_006712837.2 | XP_006712900.1 | 7 - 7 | 905 | 68 | |
| XM_011512142.2 | XP_011510444.1 | 5 - 5 | 1394 | 68 | |
| GenBank mRNA | AK057609.1 | - | 7 - 7 | 1050 | 68 |
| AK297246.1 | - | 6 - 6 | 2038 | 68 | |
| AL133619.1 | - | 3 - 3 | 2962 | 68 | |
| BC067771.1 | - | 7 - 7 | 873 | 68 | |
| CD359923.1 | - | 2 - 2 | 125 | 68 | |