The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001160300.1 | NP_001153772.1 | 1 - 1 | 200 | 68 |
| NM_052839.3 | NP_443071.2 | 1 - 1 | 200 | 68 | |
| GenBank mRNA | AF398510.1 | - | 1 - 1 | 170 | 68 |
| AK096019.1 | - | 1 - 1 | 184 | 68 | |
| AK299910.1 | - | 1 - 1 | 200 | 68 | |
| DA116770.1 | - | 1 - 1 | 202 | 68 | |
| DA127683.1 | - | 1 - 1 | 200 | 68 | |