The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_021978.3 | NP_068813.1 | 18 - 18 | 2541 | 101 |
| GenBank mRNA | AB030036.1 | - | 18 - 18 | 2363 | 101 |
| AF057145.1 | - | 18 - 18 | 2370 | 101 | |
| AF118224.2 | - | 18 - 18 | 2383 | 101 | |
| AF133086.1 | - | 18 - 18 | 2384 | 101 | |
| AK314173.1 | - | 18 - 18 | 2524 | 101 | |
| BC005826.2 | - | 11 - 11 | 1361 | 101 | |
| BC018146.1 | - | 8 - 8 | 1050 | 101 | |
| BC030532.1 | - | 18 - 18 | 2465 | 101 | |