The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001286356.1 | NP_001273285.1 | 2 - 2 | 298 | |
| NM_001286357.1 | NP_001273286.1 | 2 - 2 | 338 | 179 | |
| NM_001286358.1 | NP_001273287.1 | 2 - 2 | 247 | ||
| NM_002762.3 | NP_002753.2 | 2 - 2 | 254 | ||
| GenBank mRNA | AK130957.1 | - | 2 - 2 | 254 | |
| AK131573.1 | - | 2 - 2 | 298 | ||
| AK307320.1 | - | 2 - 2 | 338 | 179 | |
| BC042671.1 | - | 2 - 2 | 254 | ||
| BC066338.1 | - | 2 - 2 | 254 | ||
| DB066857.1 | - | 2 - 2 | 247 | ||
| X07862.1 | - | 2 - 2 | 254 | ||