The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001256799.2 | NP_001243728.1 | 5 - 5 | 560 | 120 |
| NM_001289745.1 | NP_001276674.1 | 6 - 6 | 618 | 120 | |
| NM_001289746.1 | NP_001276675.1 | 5 - 5 | 512 | 120 | |
| NM_002046.5 | NP_002037.2 | 6 - 6 | 526 | 120 | |
| GenBank mRNA | AB062273.1 | - | 6 - 6 | 390 | 120 |
| AF261085.1 | - | 6 - 6 | 441 | 120 | |
| AK026525.1 | - | 6 - 6 | 463 | 120 | |
| AK299448.1 | - | 6 - 6 | 411 | 120 | |
| AY007133.1 | - | 6 - 6 | 390 | 120 | |
| BC001601.1 | - | 6 - 6 | 392 | 120 | |
| BC004109.2 | - | 6 - 6 | 390 | 120 | |
| BC009081.1 | - | 6 - 6 | 399 | 120 | |
| BC013310.2 | - | 6 - 6 | 398 | 120 | |
| BC020308.1 | - | 5 - 5 | 376 | 120 | |
| BC023632.2 | - | 5 - 5 | 362 | 120 | |
| BC025925.1 | - | 6 - 6 | 395 | 120 | |
| BC026907.1 | - | 5 - 5 | 376 | 120 | |
| BC029340.1 | - | 6 - 6 | 384 | 120 | |
| BC029618.1 | - | 6 - 6 | 392 | 120 | |
| BC083511.1 | - | 6 - 6 | 390 | 120 | |
| BI463134.1 | - | 5 - 5 | 542 | 120 | |
| BM763361.1 | - | 6 - 6 | 503 | 120 | |
| BT006893.1 | - | 5 - 5 | 338 | 120 | |
| BU155402.1 | - | 6 - 6 | 563 | 120 | |
| CR407671.1 | - | 5 - 5 | 338 | 120 | |
| DQ403057.1 | - | 4 - 4 | 263 | 120 | |
| EF036498.1 | - | 2 - 2 | 76 | 120 | |
| EU668321.1 | - | 6 - 6 | 440 | 120 | |
| J02642.1 | - | 5 - 5 | 339 | 120 | |
| M17851.1 | - | 5 - 5 | 371 | 120 | |
| M33197.1 | - | 6 - 6 | 398 | 120 | |
| X01677.1 | - | 5 - 5 | 398 | 120 | |
| X53778.1 | - | 6 - 6 | 394 | 120 | |