The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| GenBank mRNA | AB209361.1 | - | 8 - 8 | 4271 | 121 |
| AK311164.1 | - | 7 - 7 | 802 | 121 | |
| AV715411.1 | - | 5 - 5 | 543 | 121 | |
| AY495076.2 | - | 7 - 7 | 830 | 121 | |
| BC012479.1 | - | 7 - 7 | 946 | 121 | |
| CR450307.1 | - | 7 - 7 | 636 | 121 | |
| M67454.1 | - | 7 - 7 | 830 | 121 | |
| X63717.1 | - | 7 - 7 | 856 | 121 | |
| X83490.1 | - | 5 - 5 | 414 | 121 | |
| X83491.1 | - | 4 - 4 | 351 | 121 | |
| X83493.1 | - | 6 - 6 | 598 | 121 | |
| Z47993.1 | - | 6 - 6 | 573 | 121 | |
| Z47994.1 | - | 5 - 5 | 389 | 121 | |
| Z47995.1 | - | 4 - 4 | 326 | 121 | |
| Z70519.1 | - | 6 - 6 | 527 | 121 | |
| Z70520.1 | - | 5 - 5 | 464 | 121 | |