The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_002190.2 | NP_002181.1 | 2 - 2 | 225 | 146 |
GenBank mRNA | BC066251.1 | - | 2 - 2 | 223 | 146 |
BC066252.1 | - | 2 - 2 | 223 | 146 | |
BC066253.1 | - | 2 - 2 | 223 | 146 | |
BC067503.1 | - | 2 - 2 | 223 | 146 | |
BC067504.1 | - | 2 - 2 | 223 | 146 | |
BC067505.1 | - | 2 - 2 | 223 | 146 | |
U32659.1 | - | 2 - 2 | 233 | 146 | |
Z58820.1 | - | 2 - 2 | 222 | 146 |