The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_006297.2 | NP_006288.2 | 4 - 4 | 524 | 73 |
| GenBank mRNA | AB208781.1 | - | 4 - 4 | 447 | 73 |
| AK293542.1 | - | 4 - 4 | 502 | 73 | |
| AK300163.1 | - | 3 - 3 | 416 | 73 | |
| AK315332.1 | - | 4 - 4 | 513 | 73 | |
| BC023593.2 | - | 4 - 4 | 411 | 73 | |
| BP349667.1 | - | 4 - 4 | 524 | 73 | |
| CR456728.1 | - | 4 - 4 | 404 | 73 | |
| M36089.1 | - | 4 - 4 | 952 | 73 | |