The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001101.3 | NP_001092.1 | 1 - 1 | 53 | 171 |
| GenBank mRNA | AK025375.1 | - | 1 - 1 | 53 | 171 |
| AK058019.1 | - | 1 - 1 | 53 | 171 | |
| AK130062.1 | - | 1 - 1 | 53 | 171 | |
| AK130157.1 | - | 1 - 1 | 53 | 171 | |
| AK222925.1 | - | 1 - 1 | 53 | 171 | |
| AK223032.1 | - | 1 - 1 | 53 | 171 | |
| AK223055.1 | - | 1 - 1 | 53 | 171 | |
| AK225414.1 | - | 1 - 1 | 53 | 171 | |
| AK301372.1 | - | 1 - 1 | 53 | 171 | |
| AK304552.1 | - | 1 - 1 | 53 | 171 | |
| AK309997.1 | - | 1 - 1 | 53 | 171 | |
| BC001301.1 | - | 1 - 1 | 36 | 171 | |
| BC002409.2 | - | 1 - 1 | 40 | 171 | |
| BC013380.2 | - | 1 - 1 | 44 | 171 | |
| BC013835.1 | - | 1 - 1 | 41 | 171 | |
| BC014861.1 | - | 1 - 1 | 43 | 171 | |
| BC016045.1 | - | 1 - 1 | 44 | 171 | |
| X63432.1 | - | 1 - 1 | 42 | 171 | |