The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001348450.1 | NP_001335379.1 | 1 - 1 | 33 | 81 |
| XM_015454370.1 | XP_015309856.1 | 1 - 1 | 69 | 81 | |
| GenBank mRNA | CJ443242.1 | - | 1 - 1 | 60 | 81 |