The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_026701.2 | NP_080977.2 | - | 257 | 85 |
GenBank mRNA | AK002802.1 | - | 3 - 3 | 277 | 85 |
AK143947.1 | - | 3 - 3 | 293 | 85 | |
BC019997.1 | - | - | 257 | 85 |