The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_025569.1 | NP_079845.1 | 5 - 5 | 341 | 151 |
GenBank mRNA | AK003246.1 | - | 5 - 5 | 341 | 151 |
AK003309.1 | - | 5 - 5 | 327 | 151 | |
AK003476.1 | - | 5 - 5 | 334 | 151 | |
AK003492.1 | - | 5 - 5 | 334 | 151 | |
AK008533.1 | - | 5 - 5 | 330 | 151 | |
AK135742.1 | - | 5 - 5 | 316 | 151 | |
BC029669.1 | - | 4 - 4 | 320 | 151 |