Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Brf1
Entrez Gene ID: 72308
Gene Name: BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
Gene Aliases: 2510002F24Rik, GTF3B, TAF3C, TAFIII90, TFIIIB90, mTFIIIB90
Chromosome Location: Chr.12: 112955631 - 113000707 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_028193.3	NP_082469.2	16 - 17	2081	60
GenBank mRNA	AK010890.1	-	3 - 4	330	60
	BC037224.1	-	16 - 17	1864	60
	BC058112.1	-	6 - 7	677	60