The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_207523.2 | NP_997406.1 | 2 - 2 | 84 | 125 |
| GenBank mRNA | AF452721.1 | - | 2 - 2 | 43 | 125 |
| AK028387.1 | - | 2 - 2 | 57 | 125 | |
| AK152095.1 | - | 2 - 2 | 57 | 125 | |
| BC026656.1 | - | 2 - 2 | 41 | 125 | |
| BC029892.1 | - | 2 - 2 | 43 | 125 | |
| BC086883.1 | - | 2 - 2 | 59 | 125 | |
| BC086884.1 | - | 2 - 2 | 57 | 125 | |
| BC096637.1 | - | 2 - 2 | 42 | 125 | |
| BC125640.1 | - | 2 - 2 | 63 | 125 | |
| BC125642.1 | - | 2 - 2 | 63 | 125 | |