The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
GenBank mRNA | AK004006.1 | - | 1 - 1 | 52 | 74 |
Gene Name | Gene Symbol | Alias | Distance | Relative Direction | Location Chromosome | |
---|---|---|---|---|---|---|
Upstream | pre B cell leukemia homeobox 4 | Pbx4 | 2410015M21Rik, AI429113, AW045266 | -8.1kb | Anti-sense | Chr. - 69832476 - 69872292 on Build GRCm38 |
Downstream | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 | Ndufa13 | 2700054G14Rik, AU022060, CDA016, CGI-39, GRIM-19, Grim19 | 6.8kb | Sense | Chr. - 69887788 - 69902558 on Build GRCm38 |