The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NR_111916.1 | 1 - 1 | 1689 | 97 | |
| GenBank mRNA | AK133534.1 | - | 1 - 1 | 1648 | 97 |
| Gene Name | Gene Symbol | Alias | Distance | Relative Direction | Location Chromosome | |
|---|---|---|---|---|---|---|
| Upstream | coiled-coil domain containing 81 | Ccdc81 | 4921513D09Rik | -35.0kb | Sense | Chr. - 89866148 - 89903629 on Build GRCm38 |
| Downstream | embryonic ectoderm development | Eed | ENSMUSG00000039373, l(7)5Rn, l7Rn5, lusk | 13.5kb | Sense | Chr. - 89954654 - 89981419 on Build GRCm38 |