The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_028387.1 | NP_082663.1 | 4 - 4 | 652 | 181 |
| GenBank mRNA | AK004072.1 | - | 4 - 4 | 609 | 181 |
| AK029184.1 | - | 4 - 4 | 632 | 181 | |
| BC038317.1 | - | 4 - 4 | 578 | 181 | |