The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_020030.2 | NP_064414.1 | - | 249 | 99 |
GenBank mRNA | AF257520.1 | - | 3 - 3 | 251 | 99 |
BC106190.1 | - | - | 249 | 99 |