The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001166000.1 | NP_001159472.1 | 2 - 2 | 148 | 101 |
GenBank mRNA | BC090619.1 | - | 2 - 2 | 76 | 101 |