The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001290006.2 | NP_001276935.1 | 2 - 2 | 274 | 110 |
GenBank mRNA | GT644620.1 | - | 2 - 2 | 298 | 110 |
GT873426.1 | - | 2 - 2 | 193 | 110 |