The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001105262.1 | NP_001098732.1 | 1 - 2 | 64 | 121 |
XM_027965710.1 | XP_027821511.1 | 1 - 2 | 121 | ||
GenBank mRNA | EF654537.1 | - | 1 - 2 | 63 | 121 |
EF684914.1 | - | 1 - 2 | 64 | 121 |