The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_053719.1 | NP_446171.1 | 6 - 6 | 965 | 131 |
| GenBank mRNA | AJ009698.1 | - | 6 - 6 | 965 | 131 |
| BC061846.1 | - | 6 - 6 | 967 | 131 | |
| FQ213962.1 | - | 6 - 6 | 978 | 131 | |