The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001244437.1 | NP_001231366.1 | 4 - 4 | 531 | 89 |
| XM_021068521.1 | XP_020924180.1 | - | 446 | 89 | |
| XM_021068522.1 | XP_020924181.1 | - | 273 | 89 | |
| GenBank mRNA | AK231928.1 | - | 4 - 4 | 531 | 89 |
| AK392765.1 | - | 4 - 4 | 487 | 89 | |
| AK393825.1 | - | 3 - 3 | 411 | 89 | |
| AK395083.1 | - | 4 - 4 | 484 | 89 | |
| AK398342.1 | - | 4 - 4 | 481 | 89 | |
| AK399143.1 | - | 4 - 4 | 494 | 89 | |
| AK400537.1 | - | 4 - 4 | 528 | 89 | |
| AY609757.1 | - | 4 - 4 | 477 | 89 | |
| D89492.1 | - | 2 - 2 | 152 | 89 | |