The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_021075775.1 | XP_020931434.1 | - | 2333 | 89 |
| XM_021075776.1 | XP_020931435.1 | - | 2333 | 89 | |
| XM_021075777.1 | XP_020931436.1 | - | 2093 | 89 | |
| XM_021075778.1 | XP_020931437.1 | - | 2093 | 89 | |
| XM_021075779.1 | XP_020931438.1 | - | 2161 | 89 | |
| XM_021075780.1 | XP_020931439.1 | - | 2115 | 89 | |
| GenBank mRNA | AF141957.1 | - | 4 - 4 | 352 | 89 |
| AK344046.1 | - | 16 - 16 | 2012 | 89 | |