The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_021094077.1 | XP_020949736.1 | - | 2002 | 102 |
| XM_021094079.1 | XP_020949738.1 | - | 2000 | 102 | |
| XM_021094080.1 | XP_020949739.1 | - | 2001 | 102 | |
| GenBank mRNA | AF260664.1 | - | 2 - 2 | 152 | 102 |
| AK392094.1 | - | 3 - 3 | 575 | 102 | |