The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001025214.1 | NP_001020385.1 | 3 - 3 | 244 | 113 |
| XM_005661258.3 | XP_005661315.1 | - | 586 | 113 | |
| XM_005661259.3 | XP_005661316.1 | - | 375 | 113 | |
| XM_021081930.1 | XP_020937589.1 | - | 2081 | 113 | |
| GenBank mRNA | AK231376.1 | - | 4 - 4 | 344 | 113 |
| AK345093.1 | - | 4 - 4 | 347 | 113 | |
| DQ061157.1 | - | 3 - 3 | 244 | 113 | |