The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001348949.1 | NP_001335878.1 | 3 - 3 | 231 | 110 |
| NM_213825.2 | NP_998990.1 | 3 - 3 | 231 | 110 | |
| GenBank mRNA | AF188490.1 | - | 2 - 2 | 144 | 110 |
| AK348630.1 | - | 3 - 3 | 194 | 110 | |
| AW436648.1 | - | 3 - 3 | 232 | 110 | |
| BP455542.1 | - | 3 - 3 | 190 | 110 | |
| FD607412.1 | - | 3 - 3 | 196 | 110 | |