The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_213875.1 | NP_999040.1 | 3 - 3 | 251 | 70 |
| GenBank mRNA | AF134151.1 | - | 3 - 3 | 251 | 70 |
| AK349340.1 | - | 3 - 3 | 254 | 70 | |
| M35676.1 | - | 2 - 2 | 138 | 70 | |