The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_214208.2 | NP_999373.1 | 4 - 4 | 248 | 114 |
XM_005664469.3 | XP_005664526.2 | - | 399 | 114 | |
GenBank mRNA | AB015759.1 | - | 4 - 4 | 249 | 114 |
AK350001.1 | - | 5 - 5 | 298 | 114 |