The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001098599.1 | NP_001092069.1 | 7 - 7 | 451 | 70 |
| XM_021093180.1 | XP_020948839.1 | - | 2628 | 70 | |
| XM_021093181.1 | XP_020948840.1 | - | 5126 | 70 | |
| XM_021093183.1 | XP_020948842.1 | - | 3935 | 70 | |
| XM_021093184.1 | XP_020948843.1 | - | 4153 | 70 | |
| GenBank mRNA | AK391776.1 | - | 7 - 7 | 527 | 70 |
| DQ534449.1 | - | 7 - 7 | 451 | 70 | |
| FJ719790.1 | - | 7 - 7 | 518 | 70 | |