The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001112685.2 | NP_001106155.1 | 2 - 2 | 349 | 74 |
| XM_005664042.3 | XP_005664099.1 | - | 1323 | 74 | |
| XM_005664043.3 | XP_005664100.1 | - | 580 | 74 | |
| XM_005664046.3 | XP_005664103.1 | - | 639 | 74 | |
| XM_021090863.1 | XP_020946522.1 | - | 515 | 74 | |
| XM_021090864.1 | XP_020946523.1 | - | 563 | 74 | |
| XM_021090865.1 | XP_020946524.1 | - | 627 | 74 | |
| XM_021090866.1 | XP_020946525.1 | - | 836 | 74 | |
| XM_021090867.1 | XP_020946526.1 | - | 694 | 74 | |
| GenBank mRNA | AB304400.1 | - | 2 - 2 | 351 | 74 |
| AK390264.1 | - | 2 - 2 | 362 | 74 | |