The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001112696.2 | NP_001106166.1 | 5 - 5 | 548 | 75 |
| XM_005656107.3 | XP_005656164.1 | - | 936 | 75 | |
| XM_005656108.3 | XP_005656165.1 | - | 608 | 75 | |
| GenBank mRNA | EF394366.1 | - | 5 - 5 | 550 | 75 |