The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001123178.1 | NP_001116650.1 | 9 - 9 | 652 | 92 |
| XM_013989962.2 | XP_013845416.1 | - | 995 | 92 | |
| XM_013989963.2 | XP_013845417.1 | - | 1280 | 92 | |
| XM_013989965.2 | XP_013845419.1 | - | 1072 | 92 | |
| XM_013989966.2 | XP_013845420.1 | - | 990 | 92 | |
| XM_013989967.2 | XP_013845421.1 | - | 1275 | 92 | |
| GenBank mRNA | AK231635.1 | - | 10 - 10 | 948 | 92 |
| AK236728.1 | - | 10 - 10 | 856 | 92 | |
| AK347296.1 | - | 10 - 10 | 929 | 92 | |
| AK389435.1 | - | 10 - 10 | 869 | 92 | |
| AK395489.1 | - | 10 - 10 | 969 | 92 | |
| AY610189.1 | - | 7 - 7 | 681 | 92 | |
| DQ917631.1 | - | 9 - 9 | 652 | 92 | |